医学教育改革系列教材
2012-8
张力平 高等教育出版社 (2012-08出版)
张力平
135
This laboratory text is used to guide the students'learning and training of microbiology，parasitology and immunology lab techniques，procedures and experiments.The outcome expected is students'capacity of doing research in laboratory of microbiology， parasitologyand immunology. The manual consists of two parts：PartⅠ——outlines the 8 basic experiments.PartⅡ——outlines the 21 comprehensive experiments of microbiology，parasitology and immunology.The involved experiments are designed to help enhance and deepen the students'un-derstanding of microbiology，parasitology and immunology.
Part 1 Basic Experiments Experiment 1 Observation of Bacterial Morphology Ⅰ. Using Oil Immersion I.ens of the Microscope Ⅱ. Basic Morphology and Special Structure of Bacteria Experiment 2 Bacterial Staining and Microscopy Ⅰ. Gram Stain Ⅱ. Acid-fast Stain （Ziehl-Neelsen Method） Experiment 3 Preparation of the Basic Medium Experiment 4 Examination Methods of Parasitic Infections Ⅰ. Direct Fecal Smear Method for Detecting Ascaris Eggs Ⅱ. Examination for Ascaris Larvae in Lung Tissue of Mice Ⅲ. Saturated Brine Flotation Method for Detecting Hookworm Eggs Ⅳ. Fecal Culture Method for Hookworm Larvae Ⅴ. Cellophane Tape Method for Detecting Pinworm Eggs Ⅵ. Direct Sputum Smear or Centrifugal Sedimentation Method for Detecting Paragonimus Eggs " Ⅶ. Squash Method for Detecting Striated Muscle Larvae of Trichinella Ⅷ. Test of Concentration of Eggs by Sedimentation for Detecting S. Japonicum Eggs Ⅸ. The Miracidium Hatching Method for Detecting S. Japonicum Ⅹ. Circumoval Precipitation Test （COPT） for Diagnosing Schistosomiasis Ⅺ. Direct Stool Smear with Iodine-stain for Detecting Amoeba Cysts Ⅻ. Blood Smear Method for Detecting Malarial Parasites Experiment 5 Agglutination Reaction Ⅰ. Direct Agglutination Reaction -Identification of the ABO Blood Group System Ⅱ. Indirect Agglutination Reaction -Anti-streptolysin O Test Ⅲ. Indirect Agglutination Inhibition Reaction-Latex Pregnancy Test Experiment 6 Precipitation Reaction Ⅰ. Single Radial Immunodiffusion Test Ⅱ. Double Immunodiffusion Test Experiment 7 Enzyme-linked Immunosorbent Assay （Sandwich ELISA Assay） Part 2 Comprehensive Experiments Unit 1 Isolation of Microorganisms from the Environment and Human Body Experiment 8 Bacteria in the Air and Ultraviolet Radiation Experiment 9 Bacteria on the Finger Tip and Solid Surface Unit 2 Detection of Skin Infections Experiment 10 Cultural Characteristics of Bacteria and Fungi （Teaching Demonstration） Ⅰ. Clostridium Perfringens in the Milk Medium Ⅱ. Fungi in Sabouraud Dextrose Medium Experiment 11 Observation of Plasmodium, Toxoplasma Gondii , Trichinella Spiralis ,Echinococcus Granulosus and Insecta Ⅰ. Plasmodium Ⅱ. Toxoplasma Gondii Ⅲ. Trichinella Spiralis Ⅳ. Echinococcus Granulosus Ⅴ. Insecta Unit 3 Detection of Respiratory Infections Experiment 12 Isolation & Identification of Pyogenic Cocci Ⅰ. Isolation and Culture of Mimic Pus Swab and Throat Swab Ⅱ. Coagulase Test Experiment 13 Cultural Characteristics of Respiratory Infected Bacteria Ⅰ. The Colonies of Pathogenic Cocci on Blood Agar Plates（Teaching Demonstration） Ⅱ. Mycobacterium Tuberculosis in Lowenstein-Jensen Solid Medium（Teaching Demonstration） Experiment 14 Observation of Paragonimus Westermani Unit 4 Detection of Enteric Infections Experiment 15 Isolation and Identification of Pathogenic Enterobacteria Ⅰ. Isolation of Artificial Fecal Specimens Ⅱ. Biochemical Activities of Enteric Pathogens--Kligler Iron Agar Test Ⅲ. Serologic Identification of Enteric Pathogens Experiment 16 Widal Test Experiment 17 Observation of Ascaris lumbricoides, Hookworms,Enterobius Vermicularis,Taenia ,Entamoeba and Cryptosporidium Ⅰ. Ascaris Lurnbricoides Ⅱ. Hookworms （Ancylostoma Duodenale and Necator Americanus ） Ⅲ. Enterobius Vermicularis Ⅳ. Intestinal Cestodes （Taenia Solium and Taenia Saginata） Ⅴ. Intestinal Protozoa （ Entamoeba Histolytica and Entamoeba Coli ） Ⅵ. Cryptosporidium Unit 5 Detection of Hepatic Infections Experiment 18 Observation of Clonorchis Sinensis Experiment 19 Observation of Schistosoma Japonicum Unit 6 Detection of Genitourinary Tract Infections Experiment 20 Observation of Chlamydia Trachomatis Inclusion Unit 7 Antimicrobial Resistance Experiment 21 Bacterial Conjugation:the Transfer of Plasmids Coding for Antibiotic Resistance Experiment 22 Antibiotic Sensitivity Test Unit 8 Immune Cells Function Assay Experiment 23 Macrophage Phagocytosis Assay Experiment 24 Neutrophil Phagocytosis Assay Experiment 25 Plaque Forming Cells Assay （PFC Assay） Experiment 26 Lymphocyte Transformation Assay Experiment 27 Classification of T Cell Subset and FACS Analysis Experiment 28 Cytokine Measurement by ELISPOT Appendix Appendix 1 Commonly Used Dye and Staining Appendix 2 Media Appendix 3 Commonly Used Reagents and Buffers Index
版权页： 插图： Ⅱ. Examination for Ascaris Larvae in Lung Tissue of Mice • Materials 2 % formalin, filter paper, scissors, incubator, glass slides, microscope. • Procedures （1） Cultivation of infective eggs Cover the culture dish with one layer of filter paper moistened with 2%formalin. Dissect female Ascaris, take out about 1.5 cm of one uterus nearthe vagina with forceps and then put all fertilized ova onto the culture dishevenly. Incubate the culture dish in incubator at 25℃ for 3 weeks and keep itmoisture. （2） Mouse inoculation Three weeks post cultivation, the ova are mature. Let the mouse starvedfor one day before inoculation. After that, feed the mouse with the incubatedova mixed with food. The mouse will be infected. （3） Detecting lung tissue of infected mouse From seven to twelve days after inoculation, sacrifice and dissect the in-fected mouse and take out the lungs. Cut a piece of lung tissue with scissorsand press it between two glass slides and observe the live Ascaris larvae under microscope. Ⅲ. Saturated Brine Flotation Method for Detecting Hookworm EggsHookworm eggs can float on the surface of saturated saline solution, as thegravity of saturated saline solution is bigger than that of the eggs. There-fore, this method is the best one for detecting hookworm eggs. And also,eggs from other nematodes, such as Ascaris and Trichuris can be detectedby this method. • Materials Fecal sample, saturated salt solution（specific gravity 1.2）,a small peni-cillin vessel, sticks, glass slid, cover slip, microscope. • Procedures （1） Add a little amount of saturated salt solution into a small penicillin vessel. （2） Pick appropriate quantity of fecal sample（the size as one pea）with astick into the penicillin vessel containing saturated salt solution, and mix them thoroughly. （3） Add saturated salt solution to the penicillin vessel until it is full. Place a glass slide to cover the brim of the vessel. （4） Leave the vessel for 15- 20 minutes,lift off the glass slide and re-verse it quickly,then place a cover slip on it, and examine under the micro-scope.
《医学教育改革系列教材:病原生物学与免疫学实验》由高等教育出版社出版。
此书基本上还可以，就是内容有点少，不够系统