植物病害分子流行学导论
2007-9
中国农业大学出版社
骆勇，马忠华，马占鸿 著
208
340000
《植物病害分子流行学导论（英文版）》主要内容包括：Protein—based marker：Allozymes、DNA band—based markers、DNA sequence、References and suggested readings、CltAPTER 3 PCR—BASED TECHNIQUES、Classical PCR、Allele—specific PCR、Nested PCR、Multiplex PCR等等。
PART I INTRoDUCTION CHAPTER 1 INTRoDUCl’ION TO MoLECULAR EPIDEMIOLoGY 1—1 Basic concept of molecular epidemiology 1—2 Why molecular epidemiology 1—3 Comparisons between classical and molecular methods in epidemiology 1—4 Working approaches of molecular epidemiology 1—5 About the book References and suggested readingsPARTⅡMoLECULAR TECHNIQUES USED IN EPIDEMIOLOGY CHAPTER 2 MOLECULAR MARKERS 2—1 Protein—based marker：Allozymes 2—2 DNA band—based markers 2—3 DNA sequence References and suggested readings CltAPTER 3 PCR—BASED TECHNIQUES 3—1 Classical PCR 3—2 Allele—specific PCR 3—3 Nested PCR 3—4 Multiplex PCR References and suggested readings CHAPTER 4 QUANTITATIVE REAL‘TIME PCR 4—1 Introduction 4—2 Basic real—time PCR methods 4—3 Primer and probe design for real—time PCR 4—4 Result analysis 4—5 Applications of real—time PCR References and suggested readings CHAmR 5 PRIMER I)ESIGN 5—1 Some basic concepts for primer design 5—2 Computer programs used for primer design 5—3 An example of primer design using a computer program 5—4 Degenerated PCR primersPARTⅢ MoLECULAR EPIDEMIOLOGY oF PLANT DISEASES CHAPTER 6 PATHoGEN IDENTIFICATIoN AND DISEASE DIAGNoSIS 6—1 Introduction 6—2 Use of species—specific genes or DNA regions for developing PCR primers 6—3 Use of anonymous unique DNA regions for developing PCR primers 6—4 PCR inhibitors Summary References and suggested readings CHAPTER 7 FUNGAL PATHoGEN REPRoDUCTIVE MoDES 7—1 Frequencies of genotypes 7—2 Deviation from Hardy—Weinberg expectations 7—3 Parsimony Tree Length Permutation(PTLPT) 7—4 Index of association(IA) References and suggested readings CHAPTER 8 DETEMINATIoN AND QUANTIFICATION OF INITIAL INOCULUM OF EPIDEMICS 8—1 Introduction 8—2 Molecular methods for quantification of initial inoculum 8—3 Examples of quantification of initial inoculum using molecular methods Remarkable conclusion References and suggested readings CHPTER 9 TEMpORAL AND SPATIAL DEVELOPMENT oF PATHoGEN POPULATIoNS 9—1 Introduction 9—2 Molecular approaches 9—3 Monitoring pathogen population structures 9—4 Studying spatial—temporal dynamics of pathogen populations 9—5 Providing information needed for disease management Summary References and suggested readings CHAPTER 10 PATHoGEN LONG—DISTANCE DIsPERSAL AND MIGRATION 10—1 Introduction 10—2 Pathogen lon9—distance dispersal and disease epidemics 10—3 Traditional methods to determine or infer pathogen lon9—distance dispersal 10—4 Molecular methods to study pathogen lon9—distance dispersal 10一5 Examples Summary ……PART IV METHODOLOGY OF DATA ANLYSISINDEX
