从基因到基因组
2004-1
科学出版社发行部
J.W.戴尔
359
454000
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基因技术、遗传工程、重组DNA和基因克隆等技术的快速发展，把分子生物学推到了生命科学的前沿。本书对分子生物学核心技术的原理及其使用进行了简明扼要的阐释。以对分子生物学的基本概念的简短介绍开篇，然后介绍核心的分子生物学方法及其整合运用，从单个基因的克隆与研究到整个基因组的测序，以及基因组信息的分析。最后，本书还介绍了这些技术在生物技术、医药和农业以及整个生命科学研究中的应用。 本书内容涵盖了分子生物学基础、基因克隆、核酸的纯化与分离、DNA的剪切与连接、载体、基因组文库和cDNA文库、聚合酶链反应、DNA测序、序列数据分析、基因变异分析、基因表达分析、基因功能分析、医药应用、转基因等等方面。 本书适于生物化学、分子生物学、遗传学、细胞生物学、生物技术、生物工程等相关学科及研究领域的本科生、研究生及教学科研人员参考使用。
Preface1 Introduction2 Basic Molecular Biology 2.1 Nucleic Acid Structure 2.2 Gene Structure and Organization 2.3 Information Flow:Gene Expression3 How to Clone a Gene 3.1 What is Cloning? 3.2 Wverview of the Procedures 3.3 Gene Libraries 3.4 Hybridization 3.5 Polymerase Chain Reaction4 Purification and Separation of Nucleic Acids 4.1 Extraction and Purification of Nucleic Acids 4.2 Detection and Quantitation of Nucleic Acids 4.3 Gel Electrophoresis5 Cutting and Joining DNA 5.1 Restriction Endonucleases 5.2 Ligation 5.3 Alkaline Digests 5.4 Double Digests 5.5 Modification of Restriction Fragment Ends 5.6 Other Ways of Joining DNA Molecules 5.7 Summary6 Vectors 6.1 Plasmid Vectors 6.2 Vectors Based on the Lambda Bacteriophage 6.3 Cosmids 6.4 M13 Vectors 6.5 Expression Vectors 6.6 Vectors for Cloning and Expression in Eukaryotic Cells 6.7 Supervectors:YACs and BACs 6.8 Summary7 Genomic and cDNA Libraries 7.1 Genomic Libraries 7.2 Growing and Storing Libraries 7.3 cDNA Libraries 7.4 Random,Arrayed and Ordered Libraries8 Finding the Right Clone 8.1 Screening Libraries with Gene Probes 8.2 Screening Expression Libraries with Antibodies 8.3 Rescreening 8.4 Subcloning 8.5 Characterization of Plasmid Clones9 Polymerase Chain Reaction(PCR) 9.1 The PCR Reaction 9.2 PCR in Practice 9.3 Cloning PCR Products 9.4 Long-range PCR 9.5 Reverse-transcription PCR 9.6 Rapid Amplification of cDNA Ends(RACE) 9.7 Applications of PCR10 DNA Sequencing 10.1 Principles of DNA Sequencing 10.2 Automated Sequencing 10.3 Extending the Sequence 10.4 Shotgun Sequencing:Contig Assembly 10.5 Genome Sequencing11 Analysis of Sequence Data 11.1 Analysis and Annotation 11.2 Databanks 11.3 Sequence Comparisons12 Analysis of Genetic Variation 12.1 Nature of Genetic Variation 12.2 Methods for Studying Variation13 Analysis of Gene Expression 13.1 Analysing Transcription 13.2 Comparing Transcriptomes 13.3 Methods for Studying the Promoter 13.4 Translational Analysis14 Analysis of Gene Function 14.1 Relating Genes and Functions 14.2 Genetic Maps 14.3 Relating Genetic and Physical Maps 14.4 Linkage Analysis 14.5 Transposon Mutagenesis 14.6 Allelic Replacement and Gene Knock-outs 14.7 Complementation 14.8 Studying Gene Function through Protein Interactions15 Manipulating Gene Expression 15.1 Factors Affecting Expression of Clonde Genes 15.2 Expression of Cloned Genes in Bacteria 15.3 Expression in Eukaryotic Host Cells 15.4 Adding Tags and Signals 15.5 In vitro Mutagenesis16 Medical Applications,Present and Future 16.1 Vaccines 16.2 Detection and Identification of Pathogens 16.3 Human Genetic Diseases17 Transgenics 17.1 Transgenesis and Cloning 17.2 Animal Transgenesis and its Applications 17.3 Transgenic Plants and their Applications 17.4 SummaryBibliographyGlossaryIndex
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