基因X
2010-1
高等教育出版社
Krebs
930
无
New data are acquired daily， and new insights into well-studied processes come on a scale measured in weeks or months rather than years. Its difficult to believe that the first complete organism genome sequence was obtained less than fifteen years ago. The structure and function of genes and genomes and their associated cellular processes are sometimes elegantly and deceptively simple but frequently amazingly complex， and no single book can do justice to the realities and diversities of natural genetic systems. This book is aimed at advanced students in molecular genetics and molecular biology. In order to provide the most current understanding of the rapidly-changing subjects in molecular biology， we have enlisted twenty-one scientists to provide revisions and content updates in their individual fields of expertise. Their expert knowledge has been incorporated throughout the text. Much of the revision and reorganization of this edition follows that of the second edition of Lewiss Essential GENES， but there are many updates and features that are new to this book. Most notably， there are two new chapters： Chapter3 （"Methods in Molecular Biology and Genetic Engineering"） provides an introduction to the concepts and practice of laboratory techniques in molecular biology early on in the book， and Chapter 8 （"Genome Evolution"） combines， expands， and updates material that had been scattered among various chapters in previous editions， as well as introducing a number of topics new to this book. This edition is generally up dated and reorganized for a more logical flow of topics， and many chapters have been renamed to better indicate their contents. In particular， discussion of chromatin organization and nucleosome structure now precedes the discussion of eukaryotic transcription， because chromosome organization is critical to all DNA transactions in the cell， and current research in the field of transcriptional regulation is heavily biased toward the study of the role of chromatin in this process. The discussion of transcriptional activation and chromatin remodeling has accordingly been combined into one chapter （Chapter 28）. Two chapters on transposons and retroposons have been combined into one （Chapter 17）. In addition， some chapters have been revised to contain extensive new material. The original intro ductory chapter on messenger RNA has been entirely rewritten to cover more advanced topics （Chapter 22， "mRNA Stability and Localization"）， and the regula tory RNA chapter has been dramatically expanded to include material on RNAi pathways （Chapter 30，"Regulatory RNA"）. Many new figures are included in this book， some reflecting new developments in the field， particularly in the topics of chromatin structure and function， epigenetics， and regulation by noncoding and micro RNAS in eukaryotes.
分子生物学与分子遗传学领域正经历着日新月异的变化，每天都会出现新的数据，那些热门的研究进程，过去每隔数年才会出现新的见解和看法，现在只要几周或几个月。在过去几十年里，对广大教学者来说，Lewin的《基因》是一本十分优秀的教材，该书对分子生物学和分子遗传学进行了精彩的论述，内容涵盖了基因的结构、序列、组织和表达。最新版的Lewin本书，拥有一支崭新的知识渊博的作者队伍，21位科学家根据各自的专业研究特长，对书中内容进行了修订和更新，以保证本书是本领域最新颖全面的教材。本书在内容上增加了一些新的章节，结构也进行了一些调整，使得全书各个主题在排列上更加富有逻辑性。另外许多章节也重新命名，和内容更加相符。新版中还包含了一些新的教学特色，便于学生在阅读本书过程中更好地学习；增加一个在线学习导航，学生可以使用它对关键内容进行自我测试。 新版特色 ·全新的第3章——分子生物学和基因工程方法，详细介绍了分子生物学实验技术的概念和实践。 ·新插入的第8章——基因组进化，对于早期版本分散在各章节中的相关材料做了整合、扩展和更新，并介绍了一些新进展。 ·第22章——mRNA的稳定性和定位，完全更新并重写，以包含更多的前沿内容。 ·第30章——调控RNA和小RNA，特别引入了RNAi通路的相关内容。 ·大量崭新的精美插图反映了相关领域的新进展，尤其是基闪组结构和功能、表观遗传学，以及原核生物中非编码和小RNA的调控。
ContentsPrefacePart 1. GENES AND CHROMOSOMES 1 Chapter 1. Genes Are DNA 2 Chapter 2. Genes Code for Proteins 26 Edited by Esther Siegfried, Pennsylvania State University, Altoona Chapter 3. Methods in Molecular Biology andGenetic Engineering 42Edited by John Brunstein, University of British Columbia Chapter 4. The Interrupted Gene 79Edited by Donald Forsdyke, Queens University Chapter5. The Content of the Genome 98 Chapter 6. Genome Sequences and GeneNumbers 118 Chapter 7. Clusters and Repeats 139 Chapter 8. Genome Evolution 159 Chapter 9. Chromosomes 189Edited by Hank W. Bass, Florida State University Chapter 10. Chromatin 220Part 2. DNA REPLICATIONAND RECOMBINATION 262 Chapter 11. The Replicon 263Edited by Stephen D. Bell, Oxford University Chapter 12. Extrachromosomal Replicons 282Edited by Sgren Johannes Serensen & lars Hestbjerg Hansen, University of Copenhagen Chapter 13. Bacterial Replication Is Connectedto the Ceil Cycle 299Edited by Barbara Funnell, University of Toronto Chapter 14. DNA Replication 320Edited by Peter Burgers, Washington University Medical School Chapter 15. Homologous and Site-SpecificRecombination 348Edited by Hannah L. Klein & Samantha Hoot, New York University Langone Medical Center Chapter 16. Repair Systems 391 Chapter 17. Transposable Elementsand Retroviruses 419Edited by Damon Lisch, University of California,Berkeley Chapter 18. Somatic Recombination andHypermutation in the Immune System 458Edited by Paolo Casali, Institute for Immunology,University of California, IrvinePart 3. TRANSCRIPTIONAN D POSTrRANSCRIPTIONALMECHANISMS 503 Chapter 19. Prokaryotic Transcription 504Edited by Richard Gourse, University of Wisconsin, Madison Chapter 20. Eukaryotic Transcription 546 Chapter 21. RNA Splicing and Processing 573Edited by Xiang-Dong Fu, University of California,San Diego, School of Medicine Chapter 22. mRNA Stability andLocalization 618Edited by Ellen Baker, University of Nevada, Reno Chapter 23. Catalytic RNA 642Edited by Douglas ,7. Briant, University of Victoria Chapter 24. Translation 665Edited by Cheryl Keller Capone, Pennsylvania State University Chapter 25. Using the Genetic Code 704Edited by John Perona, University of California, Santa BarbaraPart 4. GENE REGULATION 734 Chapter 26. The Operon 735Edited by Liskin Swint-Kruse, University of Kansas School of Medicine Chapter 27. Phage Strategies 767 Chapter28.Eukaryotic TranscriptionRegutation 795 Chapter 29. Epigenetic Effects AreInherited 828Edited by Trygve ToUefsbol, University of Alabama, Birmingham Chapter 30. Regutatory RNA 861Gtossary 881Index 905
Exons act as modules for building genes thaiare tried out in the course of evolution in various combinations （see Section 4.9, Some ExonCan Be Equated with Protein Functional Domains）At one extreme, an individual exon from onegene may be copied and used in another geneAt the other extreme, an entire gene, includingboth exons and introns, may be duplicated. Insuch a case, mutations can accumulate in onecopy without elimination by natural selectionas long as the other copy is under selection tcremain functional. The selectively neutral copymay then evolve to a new function, becomeexpressed at a different time or in a differenlcell type from the first copy, or become a nonfunctional pseudogene. FIGURE 8.19 summarizes our present view otthe rates at which these processes occur. Thereis a probability that a given gene will beincluded in a duplication in a period of onemillion years. After the gene has duplicated,differences evolve as the result of the occurrence of different mutations in each copy. Theseaccumulate at a rate of -0.1% per million years（see Section 8.4, A Constant Rate of Sequence Divergence Is a Molecular Clock）. If this does not happen, one of the genes is likely to become a pseudogene because it will by chance gain a deleteri ous mutation, and there will be no puri fying selection to eliminate this copy so by genetic drift the mutant version may increase in frequency and fix in the species. Typically this takes 4 mil lion years for globin genes; in general, the time to fixation of a neutral mutant depends on the generation time and the effective population size, with genetic drift being a stronger force in smaller populations. In such a situation, it is purely a matter of chance which of the two copies becomes inactive. （This can contribute to incompatibility between different individuals, and ultimately to speciation, if different copies become inactive in different populations.） Analysis of the human genome sequenceshows that -5% of the genome comprisesduplications of identifiable segments rangingin length from 10 to 300 kb. These duplicationshave arisen relatively recently; that is, there hasnot been sufficient time for divergence betweenthem for their homology to become obscured.They include a proportional share （-6%） of theexpressed exons, which shows that the duplications are occurring more or less irrespectiveof genetic content.
《基因X》由教育部高等教育司推荐，是国外优秀生命科学教学用书。
无
很好很经典的一本书。虽然有人说基因十没有基因九系统和权威，但我觉得基因十将整本书划分成几大斑块，如基因复制与重组、转录和转录后调控机制等，让人在学习时能够更加系统的了解分子生物学各领域的基本情况。尤其让我这个刚涉足分子生物学领域的学生高兴的是基因十中专门有一章介绍分子生物学常用实验（如克隆、核酸检测、DNA测序、Blotting和基因敲除等）的基本原理。总之，这本书是分子生物学领域人士的必备参考书！
分子生物学的“圣经”再升级，经典读物不用多说
值得收藏，学习分子生物的朋友应作为工具书
对于学生物的孩子来说，从基因VIII到基因X，一直都是仰视的著作吧~~~~书本的质量相当不错！值得购买！
生物学的圣物啊~~~~
学分子生物学必须要看的经典著作，英文原版，要努力啃下来，相信一定收获很多
书真的很好，跟得上最新进展。
超棒超喜欢！印刷精美~质量一流~纸张好 印刷好 内容更好！爽YY！
本书的意义就不多说了，学生物的应该都知道。
送来的书质量很好，铜版彩页，还挺重。
全英文，需要慢慢研读。
三百多元能买到跟原版几乎一样的书，很值，而且内容也确实不错。通读不现实，还是需要时查查比较好。
比中国人写的的肯定要好，适合生物爱好者，另外心疼钱的话买之前先去图书馆看看有没有，我就是买了然后在图书馆发现GeneⅩ。。。。
书挺厚的,虽然全英文可是也不难读懂,很不错,值得学生物的人人手一本.
很好,相当不错的一本书
送货速度很快，就是书皮有点小磨损，但没多大影响，当然，希望当当这方面能做得更好
书比较好，买来了之后，但太厚了，看不完基本上
发货很快，3天就从北京发到深圳。书的确很好，铜版纸印刷，很厚重，唯一的缺点就是，为啥不弄个硬壳的包装，否则怕很容易散架
做自然科学的必备之书。
适合专业人士人手一本
书不错 就是有点沉
A good reference book.
perfect choice, perfect service!
一本生物学转业的圣经，印刷精美，清晰！比原版低很多的价格，对得起这个价钱了！不过送货的速度太慢了。。。我等了一周左右才送到。
经典图书，质量不错。就是有一个小疑问，是否附带光盘？？
印刷很精美的
很好的一本书，生命科学必读。
书页有点皱，不过不影响阅读，送货速度比较快。书的价格有点贵，不过确是一本好书。
特价买的，主要是便宜
国产书也就这样
很满意 送货速度超级快
学生物同学的好书啊
基因VIII的最先修订本
经典，不需要多说
内容好，质量也可以。
分子生物学的经典之作！