生物技术概论
2012-8
科学出版社
王武 编
356
《双语教材：生物技术概论》参考国内外学科进展，结合多年教学实践过程中积累的教学资源，针对工科为主的生物工程学科特点，选择贴近生物技术产业的科技基础内容，构成了由基因工程、细胞工程、酶工程，发酵工程四个板块（篇）组成的内容体系，每篇再以章、节、小节对有关内容进行阐述。讲义中还配有图表、附录、题库、电子教材、网站等有助于学生理解和应用的信息内容和形式；形成了立体化的教学资源。
PrefacePart I Gene CloningChapter 1 Introduction to Gene Cloning1.1 Brief Overview1.2 Historical Events1.3 Significance and AlertnessChapter 2 Gene and Genome2.1 Discovery of Hereditary Factor2.2 Basic Knowledge of Gene2.3 Sequence Signals Involved with Gene Expression2.4 About Genomic and Metagenomic StudiesChapter 3 Basic Techniques for Experiments with DNA3 1 Enzymatic Tools for Gene Manipulation3.20ligonucleotide Tools3.3 DNA Purification3.4 Electrophoresis3.5 In Vitro Synthesis of DNA3.6 DNA Sequencing3.7 Macromolecular Hybridization and Blotting3.8 PCR TechniquesChapter 4 Cloning Procedure4.1 Basic Procedure of Cloning4 2 Selection of Cloning Vecto 4.3 Preparation of Donor DNA4.4 Joining of Donor and Vector by Ligation4.5 Selection Host Cell4.6 Introduction of Recombinant DNA into Host Cells4.7 "Selection, Screening and Subcloning4.8 Derivative Technology Based on CloningChapter 5 Application and Impacts5.1 Initiation of Recombinant DNA Industry5.2 General Aspects of Application5.3 Contribution and ImpactPart II Cell EngineeringChapter 6 Introduction on Cell Engineering6.1 Brief Overview6.2 Essentials of Cell Engineering6.3 Progress CluesChapter 7 Cell Fusion and Hybridization7.1 General Aspects7.2 Protoplast Technique7.3 Basic Methods of Cell Fusion7.4 Fusant Screening7.5 Application of Cell FusionChapter 8 Plant Tissue and Cell Culture8.1 Plant Tissue Culture System8.2 Gene Tra fer to Plants8.3 Artificial Seeds and Virusfree PlantsChapter 9 Animal Tissue and Cell Culture9.1 Establishment of Animal Tissue Culture System9.2 Gene Tra fer to Animal Cell9.3 Animal Hybrids and Hybridoma9.4 Approaches of Stem Cell ResearchChapter 10 Application Examples from Cell Engineering10.1 Bioproducts from Cell Engineering10.2 Breeding of New Agronomic Varieties10.3 Tra genic Animals as Models for Medical ResearchPart III Enzyme TechnologyChapter 11 Fundamental Nature of Enzyme11.1 Definition, Basic Nature and Classification of Enzyme11.2 Determination of Enzyme Activities11.3 Facto Affecting Enzyme Activity11.4 Resources of Enzyme11.5 Uses of EnzymeChapter 12 Enzyme Biosynthesis and Products12.1 BiDresources of Enzyme12.2 About Enzyme Synthesis12.3 Enzyme Fermentation and ProductsChapter 13 Enzyme Recovery& Purification13.1 Pretreatment of Crude Enzyme Sample13.2 Removing of Impurities and, Sample Concentration13.3 Chromatographic Separation13.4 Polishing of Enzyme13.5 Rational Formulation of Final Enzyme ProductsChapter 14 Enzymatic Conve ion14.1 Enzymatic Conve ion14.2 Immobilization of Enzyme and Cells14.3 Enzymatic conve ion in nonaqueous PhaseChapter 15 New Approaches of Enzyme Technology15.1 Modification on Enzymes15.2 Enzyme Carrie for Immobilization15.3 Enzyme Inhibito 15.4 Ribozyme （Nonprotein Enzymes）Part IV Industrial FermentationChapter 16 Principles of Fermentation16.1 Fermentation Principles16.2 Fermentation Products and Application16.3 Benefits from the Fermentation IndustryChapter 17 Industrial Microorganisms17.1 Types and Characte of Industrial Microorganisms17.2 Breeding of Industrial Strai 17.3 Industrial Microbial Growth and Culture Media17.4 Inoculation of Industrial Strai 17.5 Preservation of Industrial Strai Chapter 18 Microbial Metabolism and Fermentation Kinetics18.1 Metabolism Related toBasic Pathway18.2 Metabolic Regulation and Control18.3 Stoichiometry of Fermentation Process18.4 Fermentation KineticsChapter 19 Bioreacto and Auxiliary Equipments19.1 Structure and Function of Bioreacto 19.2 Auxiliary Equipment19.3 Probes and Se o Chapter 20 Fermentation DownStream Technology20.1 General Aspects20.2 Harvest and Pretreatment20.3 Isolation and Purification20.4 Product Polishing20.5 Waste Treatment and Cleaner ProductionChapter 21 Progress in Fermentation Engineering21.1 Approaches of Upstream Technology21.2 Process Optima21.3 Development of Dow tream TechnologyAppendixTerms Tra lationRelated Nobel LaureatesFurther Reading后记
